Capabilities:
– Upright configuration Wide Field Microscope
– Bright Field Imaging with Dark Field option
– Phase contrast and DIC imaging
– Widefield fluorescence microscopy
– Motorized fluorescence cube filter wheel, objective lens, and condenser lens
– Fully motorized stage (XYZ) ideal for image stitching, tilting and Z-stacking
– Both Color & High sensitivity Black& White CCD cameras
Cost:
Academic users: $15/h, Industry users: $35/h
Specifications:
Microscope:
Olympus BX53F2 upright
Light Source:
LED Transmitted light
Short Arc Mercury lamp for reflected/fluorescence excitation
Objectives:
4x/NA 0.16/Air UPLXAPO
10x/NA 0.25/Air UPLXAPO
20x/NA 0.50/Air UPLFLN PH (#1.5 cover slip)
40x/NA 0.75/Air UPLFLN PH (#1.5 cover slip)
40x/NA 0.95/Air UPLXAPO (#1.5 cover slip)
60x/NA 1.2/Water UPLSAPO (#1.5 cover slip)
60x/NA 1.35/Oil UPLSAPO (#1.5 cover slip)
Most of our other Olympus objectives can also be used, including Water Dipping objectives.
Filter Cube Sets:
- Bright Field & Phase Contrast (empty)
- DAPI, Hoechst (Ex: BP375/28, 415DC, Em: LP435)
- FITC, eGFP (Ex: BP470/40, 495DC, Em: BP525/50)
- TRITC, RFP (Ex: BP546/22, 565DC, Em: BP590/33)
- Cy5, Alexa633, 647 (Ex: BP620/60, 660DC, Em: BP700/75)
- eCFP, Alexa430 (Ex: BP436/20, 455DC, Em: BP480/40)
- eYFP (Ex: BP500/20, 515DC, Em: BP535/30)
Camera and Control:
DP80 Dual Chip Color and Monochrome Camera
Olympus CellSens Standard software on a Windows 10 PC
Stage can accommodate our temperature control and petri dish inserts.