PerkinElmer VoX Spinning Disk

Capabilities:

– High-speed multi-color image acquisition with spinning disk

– High resolution multi-color image with 6 laser lines (405, 440, 488, 514, 561 and 640 nm)

– Fast and high-resolution image acquisition with EMCCD camera (30.6 frame/s, 1000 x 1000 pixels)

Cost:

Academic users: $40/h, Industry users: $60/h

 

Specifications:

Microscope:

Leica DMi8 inverted microscope

Prior motorized stage

Yokogawa CSU-X1 spinning disk scan head

 

Illumination and Detection:

Solid-laser lines for 405, 440, 488, 514, 561 and 640 nm

Available for DAPI, CFP, GFP, Alexa488, FITC, YFP, RFP, mCherry, Propidium iodide in switchable filter wheel

Alexa405/DAPI 435-485

CFP 460-500

YFP 525-555

Alexa 488/GFP/FITC 507-543

RFP/PI 570-620

Alexa 635/Cy5 612-688

 

Objectives:

10x/NA 0.3/Air (WD 11.13mm)

20x/NA 0.7/Glycerol (WD 2.0mm)

40x/NA 1.25/Oil (WD 0.1mm)

63x/NA 1.3/Glycerol (WD 0.28mm)

100x/NA 1.4 /Oil (WD 0.09mm)

 

Camera:

Hamamatsu 9100-02 CCD camera (30.6 frame/s, 1000 x 1000 pixels) for all objectives

Hamamatsu 9100-23B CCD camera (70.4 frame/s, 512 x 512 pixels) for 100x/Oil objective

 

Computer:

Improvision Volocity software operated by Windows 7